Mesenteric lymph node IL-12p40 is produced independently of MyD88 during oral infection.

Mice were infected and tissues harvested as described in Fig 2 legend. (A) Total number of DC, PMN and MAC in mesenteric lymph nodes (MLN) from MyD88 WT and KO mice. (B) Flow cytometric analysis of IL-12p40 expression in the presence and absence of MyD88. Numbers in the scatter plots indicate the percent of cells falling within the indicated gate. (C) Frequency and (D) number of IL-12 positive cells amongst DC, PMN and MAC in the MLN. (E) Frequency of IL-12p40 expression amongst mesenteric lymph node DC subsets. (F) IL-12p40 secretion in bulk cultures of MLN cells cultured in media or stimulated with STAg. The solid line indicates IL-12p40 levels produced by noninfected KO MLN cells. Values are the means ± SEM of two independent experiments and each symbol represents an individual mouse. Open and closed symbols delineate data obtained from one independent experiment. The data were analyzed in an un-paired Student’s t test where * p<0.05.

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