MORC3-84aa attenuates IFN I signaling pathway by targeting TRIF.

(A) The qRT-PCR analysis of ISG15, Mx1, and Viperin mRNA in MsbC cells under poly(I:C) treatment. (B) The qRT-PCR analysis of ISG15, Mx1, and Viperin mRNA in MsbC cells with SCRV infection. (C) The qRT-PCR analysis of ISG15, Mx1, and Viperin mRNA in MsbC cells by poly(I:C) stimulation. (D) Relative luciferase activities were detected in MsbC after co-transfection with phRL-TK Renilla luciferase plasmid, luciferase reporters, control or Linear-Flag-MORC3-84aa under poly(I:C) stimulation. (E) Relative luciferase activities were detected in MsbC after co-transfection with phRL-TK Renilla luciferase plasmid, luciferase reporters, 100 nM NC or si-circMORC3-2 after SCRV infection. (F) Relative luciferase activities were detected in MsbC cells after co-transfected with MAVS, TRIF, MITA, TBK1, and TRAF3 expression plasmids, phRL-TK Renilla luciferase plasmid, luciferase reporters, Linear-Flag-MORC3-84aa or pcDNA3.1 plasmids. (G) Relative luciferase activities were detected in MsbC cells after co-transfected with TRIF expression plasmid, phRL-TK Renilla luciferase plasmid, luciferase reporters, pcDNA3.1 or concentration gradient of Linear-Flag-MORC3-84aa. All data presented as the means ± SE from at least three independent triplicated experiments. **, p < 0.01; *, p < 0.05 versus the controls.