MOESM9 of Initial high-resolution microscopic mapping of active and inactive regulatory sequences proves non-random 3D arrangements in chromatin domain clusters

Additional file 9. 3D nuclear topography and quantitative mapping of ~40 kb targets of DHS[−] regions in A549 nuclei. (A) Part of a SIM light-optical section from a whole nucleus acquisition with representative inset magnifications. DAPI-stained DNA after intensity classification shown as gray gradations and color heat map, respectively. Segmented signals delineating targets both of fosmid pool 1 (green) and fosmid pool 2 (red) show a similar location with regard to chromatin compaction classes (asterisks in color heat maps, pool 1 (green), pool 2 (black). Scale bar 2 µm, insets 0.5 µm. (B) Quantified distributions (N = 10 nuclei) of fosmid pools 1 (green) and 2 (red) within respective chromatin compaction classes (all classes shown in gray). (C) Quantified levels of relative enrichment (positive values) or depletion (negative values) of fosmid pool 1 and pool 2 signals show an enrichment of signals in higher compaction classes. Error bars = standard deviation of the mean *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.