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MOESM9 of Initial high-resolution microscopic mapping of active and inactive regulatory sequences proves non-random 3D arrangements in chromatin domain clusters

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posted on 2019-10-24, 13:40 authored by Marion Cremer, Volker Schmid, Felix Kraus, Yolanda Markaki, Ines Hellmann, Andreas Maiser, Heinrich Leonhardt, Sam John, John Stamatoyannopoulos, Thomas Cremer
Additional file 9. 3D nuclear topography and quantitative mapping of ~40 kb targets of DHS[−] regions in A549 nuclei. (A) Part of a SIM light-optical section from a whole nucleus acquisition with representative inset magnifications. DAPI-stained DNA after intensity classification shown as gray gradations and color heat map, respectively. Segmented signals delineating targets both of fosmid pool 1 (green) and fosmid pool 2 (red) show a similar location with regard to chromatin compaction classes (asterisks in color heat maps, pool 1 (green), pool 2 (black). Scale bar 2 µm, insets 0.5 µm. (B) Quantified distributions (N = 10 nuclei) of fosmid pools 1 (green) and 2 (red) within respective chromatin compaction classes (all classes shown in gray). (C) Quantified levels of relative enrichment (positive values) or depletion (negative values) of fosmid pool 1 and pool 2 signals show an enrichment of signals in higher compaction classes. Error bars = standard deviation of the mean *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.


Ludwig-Maximilians-Universität München