12934_2015_333_MOESM3_ESM.tif (2.16 MB)
MOESM3 of A CYP21A2 based whole-cell system in Escherichia coli for the biotechnological production of premedrol
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posted on 2015-09-15, 05:00 authored by Simone Brixius-Anderko, Lina Schiffer, Frank Hannemann, Bernd Janocha, Rita BernhardtAdditional file 3: Fig. S3. Vector maps of constructed vectors for a CYP21A2 based whole-cell system in E. coli with etp1fd as final electron donor. Two vectors with tricistronic transcription units were constructed, based on the pET17b vector with an inducible T7 promotor and an ampicillin resistance gene, with etp1fd as final electron donor in combination with the reductases arh1 (p21b_ArEt) or Fpr (p21b_FrEt).
