Lung RNA-seq.

(A) PCA plot showing the relationship between vaccinated and unvaccinated groups challenged with different variants. For each group, centroids are calculated. (B) Pairwise comparisons of the number of up and down-regulated genes between unvaccinated and vaccinated animals challenged with each variant (adj. p<0.05). (C) Heatmap showing gene expression of the top 20 genes involved in the defense response to virus (GO:0051607). (D) Heatmap of the 1,000 most significant genes. A common legend for A, C and D panels is included. Hierarchical clustering was performed through Ward’s clustering criterion. Relevant genes from cluster 1 and 2 are highlighted on the right.