posted on 2022-02-02, 18:40authored byXiaoqing Cheng, Fenglan Zhang, Jingwen Gong, Yige Li, Dan Zhou, Jing Wang, Eu Gene Vong, Ying Yuan, Maode Lai, Dandan Zhang
(A) Luciferase reporter assays of PRE1 containing rs77544449 and rs7229639 with either the major or minor allele, or empty vector (PGL4minP) were performed in HEK293T cells. (B) Luciferase reporter assays of PRE2 containing rs60385309 and rs72917785 with either the major or minor allele, or empty vector (PGL4minP) were performed in HEK293T cells. (C-F) Luciferase reporter assays of two PREs containing candidate functional SNPs with either the major or minor allele was measured in DLD-1 cells or HCT116 cells. Two-tailed Student’s t tests were used to calculate P values. Error bars, SD. n = 3. * P<0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. ns, not significant.