Loss of PrP^C in the 1C11 neuronal cell line impacts on the expression of glycolytic and mitochondrial enzymes.

(A) Relative amounts of glycolytic and mitochondrial enzymes involved in the oxidative degradation of glucose in PrP^null-1C11 cells as compared to parental 1C11 cells. (B) Relative mRNA levels of fructose-bisphosphate aldolase A (AldoA), triosephosphate isomerase (TPI), beta-enolase 3 (Eno3), phosphoglycerate mutase 1 (PGAM1), alcohol dehydrogenase (ADH), 2-oxoglutarate dehydrogenase (OGDH), citrate synthase (CS), pyruvate dehydrogenase E1 (PDHA1) and dihydrolipoyl dehydrogenase (DLD) in PrP^null- vs. 1C11 cells (n = 10). (C) Histogram quantifications for AldoA, Eno3, PGAM1, TPI, ADH, OGDH, CS, PDHA1 and DLD protein expression in 1C11 and PrP^null-1C11 cells. α-tubulin was used for normalization (n = 7). Values are mean fold change ± SEM. * denotes p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.