posted on 2021-09-15, 17:26authored byEunhye Goo, Ingyu Hwang
(A) The ObcA protein was pulled down from the total cell lysate of the wild type strain grown in the early stationary phase using anti-ObcB and anti-Lon antibodies in affinity pulldown experiments. Western blotting was performed using an anti-ObcA antibody to detect ObcA in the immunoprecipitated samples. * denote additional unidentified positive signals. (B) The ObcB protein was detected from the total cell lysate of the wild -ype strain grown in the early stationary phase using an anti-Lon antibody. Ma, Input, IP, and WB denote molecular markers, total lysate of the wild type, immunoprecipitation, and western blotting, respectively.