Local delivery of Ad-mIL-4

Copyright information:

Taken from "Gene therapy for established murine collagen-induced arthritis by local and systemic adenovirus-mediated delivery of interleukin-4"

Arthritis Research 2000;2(4):293-302.

Published online 24 May 2000

PMCID:PMC17812.

Copyright © 2000 Current Science Ltd

Three days after lipopolysaccharide treatment collagen-immunized mice were injected periarticularly with 5×10 particles of Ad-mIL-4, Ad-eGFP, or saline. Arthritis index. Subsequently, severity of disease was assessed every other day using an established macroscopic scoring system ranging from 0 to 4: 0, normal; 1, detectable arthritis with erythma; 2, significant swelling and redness; 3, severe swelling and redness from joint to digit; and 4, maximal swelling with ankylosis or necrosis. The average macroscopic score was expressed as a cumulative value for all paws, with a maximum possible score of 16 per mouse ( = 20).