pone.0262173.g002.tif (335.01 kB)

Lipid metabolic functionality in 3D human hepatic constructs.

figure

posted on 2022-01-20, 18:26 authored by L. Madison Kirk, Charlotte Mae K. Waits, Alexander C. Bashore, Beverly Dosso, Allison K. Meyers, Antonio C. Renaldo, Thomas J. DePalma, Kelli N. Simms, Nathaniel Hauser, Chia-Chi Chuang Key, Charles E. McCall, John S. Parks, Susan Sergeant, Carl D. Langefeld, Aleksander Skardal, Elaheh Rahbar

The hepatic construct was incubated with [³H]-oleic acid and [¹⁴C]-Acetic acid for 1.5 hours (n = 3) to quantify lipid esterification and lipogenesis functionality, respectively. (A) Formation of [³H]-phospholipid (PL), [³H]-triglyceride (TG), and [³H]-cholesteryl ester (CE) from of [³H]-oleic acid was measured to access the ability of fatty acid esterification. (B) De novo synthesis of [¹⁴C]-PL, [¹⁴C]-free cholesterol (FC), [¹⁴C]-TG, and [¹⁴C]-CE from [¹⁴C]-acetic acid were measured fractions. Scintillation counts were normalized to total DNA, and mean ± SEM are illustrated. DPM, disintegrations per minute.