Leaf initiation rates and morphologies of mir172 mutants the 35Spro:MIM172 line.

(A) A photograph of mir172a-2 b-3 c-1 d-3 siliques grown in LD conditions from a single plant. The ages of the siliques are in order from left (early arising) to right (late arising). Note that the phenotype is most severe in the first flowers/siliques to arise. (B) A photograph of mir172a-2 b-3 d-2 inflorescence. Note the extremely serrated cauline leaves (arrowheads). (C) Graphs depicting the TLN and DTB of Col-0, mir172c-1, mir172e-1, and mir172c-1 e-1 grown under LD conditions. (D) The number of leaves present on SD-grown plants that were larger than 0.25 cm on each indicated day. Error bars indicate SEM. (N = 12–16). Statistical comparisons relative to Col-0 at 40d: mir172a; Δ4.18 RLN; p = 0.001), (mir172ab; Δ5.03 RLN; p < 10–5), (mir172abc; Δ3.75 RLN; p = 0.003), (mir172abcd(e/+); Δ4.69 RLN; p = 0.0004). The Venus-rSPL15 genotype was used here as a control. Data underlying panel D are provided in S2 Data. (E) Micrographs of RNA in situ hybridizations using a commercial probe designed to recognize miR172 of Col-0 (upper panels) and mir172a-2 b-3 c-1 d-3 e-1 (lower panels) apices grown under SD conditions for the indicated times. (F) A representative photograph of the morphologies of plants of the same age grown under SD conditions. The genotypes used in each experiment were Col-0, mir172a-2, mir172a-2 b-3, mir172a-2 b-3 c-1, mir172a-2 b-3 c-1 d-3 e-1/+, 35Spro:MIM172, and Venus-rSPL15. DTB, days to bolting; LD, long-day; SEM, standard error of the mean; SD, short-day; TLN, total leaf number.

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