Fig 9.TIF (1.08 MB)
LC-MS/MS Confirmation of Ciguatoxins.
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posted on 2016-04-13, 05:54 authored by D. Ransom Hardison, William C. Holland, Jennifer R. McCall, Andrea J. Bourdelais, Daniel G. Baden, H. Taiana Darius, Mireille Chinain, Patricia A. Tester, Damian Shea, Harold A. Flores Quintana, James A. Morris Jr., R. Wayne LitakerLC-MS/MS chromatogram showing the retention time (4.76 min) and the three characteristic ion transitions (1123.6 > 1105.6, 1123.6 > 1087.9, and 1123.6 > 1069.6) of a C-CTX-1 standard and in a lionfish sample.
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Fish Ciguatera fish poisoningradiolabeled brevetoxindetection limitcompetition bindingCaribbean fish1.0 ppbBODIPYsite 5 receptors5 yearsreceptor binding assayR 2toxicity levelsalternative methodOther RBAsample throughputradiolabeled receptor binding assay0.075 ppbFluorescent Receptor Binding Assaylicense requirements2.5 daysCarribean ciguatoxinsradioisotope utilizationsite 5fish tissuesscreening fish samplesdata analysesuse radioisotopesCTX monitoring programs
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