LANA expressed in trans promotes KSHVΔLANA broad viral gene transcription following infection in 293FRT cells.

A. Immunoblot of LANA in 293FRT/LANA or BCBL1 PEL cells. 5x10⁴ cells were loaded per lane. (BCBL1 PEL cells have less cytoplasm than do 293FRT cells, accounting for less tubulin in the BCBL1 lane.). B-I. Ratios of viral RNA to DNA following KSHVΔLANA infection of 293FRT/LANA or 293FRT cells for the indicated viral genes at 24, 48 or 72 hpi. **, p<0.01; ***, p<0.001; ****, p<0.0001 by unpaired t test. J. Heatmap of z score values showing relative viral gene expression for all KSHV genes following KSHVΔLANA infection of 293FRT or 293FRT LANA stably expressing cells at 24 or 72 hpi. Three independent biological replicates are shown for each time point. Early lytic, late lytic, or latent classes of genes are indicated at left. Gray color indicates absent values.