Knockout of PrP^C does not affect total APP levels in wild type or AD-model mice.

A) Total brain homogenate from 12-month old mice across 8 genotypes were analysed via western blot. APP was labelled using the N-terminal monoclonal antibody 22C11, and PrP^C labelled using ICSM18. n = 5. B) Quantification of APP expression levels determined by western blot. J20 mice showed significantly higher total APP expression than APP-PS1 mice irrespective of PrP^C status (APP-PS1 vs J20, p = 0.024), however no significant differences were observed between the PrP^C +/+ and -/- variants of any APP genotype. n = 5. C) Quantification of PrP^C expression levels determined by western blot. No significant differences in PrP^C expression were observed between APP genotypes. Deletion of PrP^C resulted in a significant difference on PrP^C expression levels for all the lines used in this study, when compared to their respective controls (WT vs PrP^C KO, p = 0.036; APP-PS1 vs APP-PS1 PrP^C KO, p = 0.019; WT vs PrP^C KO, p = 0.019; J20 vs J20 PrP^C KO, p = 0.026). n = 5.