Kaplan-Meier plots of ammonium acetate-induced mortality.

Zebrafish larvae (n = 100 per group) at 1 or 4 dpf were exposed to varying concentrations (5, 10 and 20 mM) of either NH₄Ac or NaAc and survival rates monitored for up to 36 h. While NH₄Ac was not toxic to zebrafishes at 1 dpf (A), exposure at 4 dpf induced death of all larvae in a dose-dependent manner (log-rank test, P<0.001) (B). In contrast, NaAc had virtually no toxic or lethal effect at either developmental stage (C, D).