KSHV does not undergo spontaneous lytic infection following infection of NOK cells and LANA inhibits most viral gene expression.

A. Gardella gel at 24 or 96 hpi at the indicated MOI following KSHV or KSHVΔLANA infection of NOK cells. Each lane pair contains independent infections. O, gel origin; E, episomal DNA; L, linear viral genomic DNA. B-I. Ratios of viral RNA to DNA following KSHV or KSHVΔLANA infection of NOK cells for the indicated viral genes at 24, 48 or 72 hpi. ns, p>0.05; *, p<0.05, **, p<0.01; ***, p<0.001;****, p<0.0001 by unpaired t test. J. Heatmap of z score values showing relative viral gene expression for all KSHV genes following KSHV (WT) or KSHVΔLANA (ΔL) at 24 or 72 hpi in NOK cells. Three independent biological replicates are shown for each time point. Early lytic, late lytic, or latent classes of genes are indicated at left.