KSHVΔLANA does not express truncated LANA products from downstream initiation of translation.

Immunoblot was performed using anti-LANA or anti-GFP antibody following infection of 293 cells with KSHV or KSHVΔLANA, or following transient tranfection of GFP LANA 779–1162 or GFP LANA 933–1162 in 293 cells. LANA, GFP, GFP LANA 779–1162 or GFP LANA 933–1162 bands are indicated by asterisks to the right of each band. GFP is expressed from the recombinant KSHV or KSHVΔLANA genomes. Control lane contains uninfected and untransfected 293 cells. GFP LANA 779–1162 contains LANA repeat elements, accounting for increased signal with the LANA polyclonal antibody compared to GFP LANA 933–1162, despite its lower expression, as observed in the anti-GFP immunoblot.

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