ppat.1010219.s007.tif (2.02 MB)

Itaconate and DI treatments lead to a global downregulation of IFN-I signaling in IAV-infected dTHP-1 cells.

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posted on 2022-01-13, 18:46 authored by Aaqib Sohail, Azeem A. Iqbal, Nishika Sahini, Fangfang Chen, Mohamed Tantawy, Syed F.H. Waqas, Moritz Winterhoff, Thomas Ebensen, Kristin Schultz, Robert Geffers, Klaus Schughart, Matthias Preusse, Mahmoud Shehata, Heike Bähre, Marina C. Pils, Carlos A. Guzman, Ahmed Mostafa, Stephan Pleschka, Christine Falk, Alessandro Michelucci, Frank Pessler

Cells were infected with IAV (PR8M, MOI = 1) and gene expression assessed by microarray analysis 12 h p.i. Enrichment analysis of gene ontology (GO Biological Process) terms based on the microarray data shown in Fig 3, using DEGs (p<0.05, FC >|1.5|) as input. GO terms are listed in reverse alphabetical order from top to bottom (page split after “protein”), with upright (red) triangles indicating enrichment (upregulation) and downward pointing (blue) triangles depletion (downregulation). Enriched GO terms (FDR ≤0.05) reveal major induction of antiviral and inflammatory responses by infection, which are decreased by both itaconate and DI treatment (most relevant inflammation and infection related GO terms are printed in bold font).

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