Isotopologue distribution patterns for metabolites in or directly related to the TCA cycle of C. burnetii wildtype, 0823::Tn mutant and 0823::Tn pFLAG-CBU0823 complemented strains.

C. burnetii incubated with [¹³C]glucose supplemented ACCM-2 for 7 days before GC-MS analysis to determine ¹³C incorporated into the extracted metabolites. Mean isotopologue distribution of each metabolite detected, corrected for natural isotopic abundance with 0% unlabelled biomass, was graphed using DExSI for each strain. Error bars represent standard deviation from five biological replicates. Solid lines represent a single enzyme and dotted lines represent multiple enzymes. The blue line denotes the activity of CBU0823 as a ME. The green line denotes CBU1241 as an MDH. The orange line represents an unestablished conversion of pyruvate to lactate by an LDH-type reaction. PEP = phosphoenolpyruvic acid.

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