Isotope distribution of guanine and cytosine nucleotides in CVB3 infected Hela R19 cells.

¹³C-glucose isotope tracing study in mock- and CVB3-infected HeLa R19 cells (three replicates; one experiment; multiplicity of infection (MOI) = 5). Cells were infected, lysed at 2,4,6 or 8 hpi and measured by LC-MS to identify metabolites and quantify the different isotopologues. In one replicate of 6h mock, CMP was undetectable leading to inaccurate fraction calculations. Therefore, the 6h mock was omitted in the CMP figure. The p-values were calculated using linear mixed effect models with an interaction of time and treatment and a random effect of replicate. For CMP, a normal distribution of the residuals could not be assumed and therefore a non-parametric linear mixed effect model with an interaction of time and treatment and a random effect of replicate was performed. Afterwards, a contrast analysis was done to calculate the p-values between specific groups. For this analysis, the fractions of all labels were added together and tested whether the total amount of labeling differed between mock and CVB3 infection. *p < 0.05, **p < 0.01, ***p < 0.001.

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