pone.0289860.g001.tif (1.66 MB)
Isolation of baboon bone marrow erythroid cell subpopulations.
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posted on 2023-12-22, 18:38 authored by Vinzon Ibanez, Kestis Vaitkus, Maria Armila Ruiz, Zhengdeng Lei, Mark Maienschein-Cline, Zarema Arbieva, Donald LavelleA. Representative FACS plots showing separation of baboon BM erythroid cell subpopulations. B. Wright’s stained cytospin preparation of isolated BM erythroid subpopulations. C. Morphology of BFUe and CFUe colonies identified in the R6 (CD34+CD117+bRBC-) and R5 (CD34+CD117+bRBC+) subpopulations, respectively shown at 40X magnification. D. Results of methylcellulose colony assays showing enrichment of BFUe and CFUe within the various BM subpopulations (200 cells plated; mean±SD, n = 3).
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small molecule drugsickle cell diseaseprolonged treatment periodprevious work showedpapio anubis increasing life spanincluded increased levelshighly purified subpopulationshighly purified populationfetal hemoglobin interfereglobal gene expressionerythroid differentiation responsiblestimulates hbf expressionlsd1 inhibitor rnerythroid differentiationinhibitor rnglobal transcriptomehematopoietic differentiationvarying stagessilencing modelsresults showpharmacological inhibitionmethyl lysinelimited numberlarge numbersideally suitedhbf elevationsh3k4 diexist worldwideepigenetic modificationsearly eventdramatic effectsbaboons treatedbaboons targetsbaboons (<
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