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Isolated rat hindquarter preparation

figure

posted on 2011-12-30, 14:47 authored by Adrian Mondry

Copyright information:

Taken from "Antiglucocorticoid RU38486 reduces net protein catabolism in experimental acute renal failure"

BMC Nephrology 2005;6():2-2.

Published online 17 Feb 2005

PMCID:PMC550647.

The perfused muscle mass is below the dotted line. The tail is clamped off. Recirculating perfusion system. Heart piece is the oxygenator (1), wherein the medium is gassed (95% O, 5% CO) and from where it is pumped (3) up via a filter (nylon mash, 20 μm width) (2) and pH and pOprobes (4) to attain hydrostatic pressure of 80 mm Hg (5) before entering the hindquarter (6). Intermediate sampling and pH adjustment can be carried out if necessary (7).