Integrative analysis of metabolomics and phosphoproteomics confirms the importance of nucleotide metabolism in CVB3 infection.

A, B) DIABLO analysis (supervised analysis of multiple omics datasets) of the metabolomics and the total phosphoproteomics dataset (A) or the phosphoproteomics dataset filtered for metabolic proteins (B) of mock and CVB3-infected HeLa R19 cells. C) Enrichment analysis of proteins extracted from panel A using inBio discoverer (featuring Gene Ontology enrichment analysis). Fold enrichment of the major Gene Ontology (GO) terms is plotted, D) Regulated phosphosites on enzymes directly involved in nucleotide metabolism. Plotted are the log2 LFQ intensities of four replicates (one experiment).The p-values are calculated using ANOVA and subsequent Welch’s T-tests *p < 0.05, **p < 0.01, ***p < 0.001.