pntd.0009940.s005.tif (1.19 MB)
Innate cells from frozen PBMCs lose their responsiveness.
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posted on 2021-11-12, 18:36 authored by Brenda Rocamonde, Nicolas Futsch, Noemia Orii, Omran Allatif, Augusto Cesar Penalva de Oliveira, Renaud Mahieux, Jorge Casseb, Hélène DutartreA. A total of 2x106 cells from fresh or frozen PBMCs were registered and selected by cell size and granularity. After selection of single cells, viable cells were gated and innate immune cell populations were identified as indicated in S1 Fig. B. PBMCs from fresh or frozen PBMCs were stimulated with R848 and analyzed by flow cytometry for their intracellular production of IL-12 and TNFα (for BDCA3+ cDC1; BDCA1 cDC2 and monocytes) or IFNα and TNFα (for pDC).
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tropical spastic paraparesissup >+pdc producing ilcell leukemia virushigher ifnγ responsivenessdevastating neurodegenerative diseasemonocytes producing mipincluding asymptomatic carriersdiv >< ptsp patients comparedtsp blood samplesblood samplesasymptomatic carrierstsp patientstsp comparedhigher frequencydisease occurrencedisease manifestationsteady statesignaling pathwaypotential consequencenk cellsneuroinflammatory statusmechanism underlyinglower responselike receptorinflammatory responseinfected volunteersincreasing numberincreased sensitivityimmune cellseffective treatmentcorticosteroid treatmentsadaptive compartment
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