ppat.1012036.s006.tif (1008.88 kB)

Inhibition of nucleotide salvage constraints CVB3 virus production early in infection.

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posted on 2024-03-08, 18:21 authored by Lonneke V. Nouwen, Martijn Breeuwsma, Esther A. Zaal, Chris H. A. van de Lest, Inge Buitendijk, Marleen Zwaagstra, Pascal Balić, Dmitri V. Filippov, Celia R. Berkers, Frank J. M. van Kuppeveld

Growth kinetics of CVB3 in HeLa R19 cells in the presence of different concentrations of 6-MP (A, B) or CPU (C, D). The cells were infected, treated with the compounds directly after infection, lysed at 2, 4, 6, or 10 hpi and titrated on HeLa R19 cells to determine the TCID50/ml (MOI 5; mean ± SEM of triplicates; one experiment). Guanidine hydrochloride (GuaHCl) is a known replication inhibitor. A,C) Growth curve of all the included time points. B,D) Bar graph of the 4h time point. A two-way ANOVA was performed, but results are not significant.

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