Influenza infection shifts the fusion/fission molecular machinery balance towards a fusion state.

a, A549 cells infected (or mock infected) with influenza A H1N1 virus at MOI 1 for 24h were immunostained with anti-TOMM20 antibody (green), anti-DRP1 antibody (red) and DAPI (blue); arrowheads indicate recruitment of DRP1 onto the mitochondrial surface (TOMM20). b, Quantification of DRP1 signal on TOMM20 positive structures from A549 cells illustrated in (a) (N = 30 cells from three independent experiments). c, A549 cells infected (or not) with influenza A H1N1 virus at MOI 1 for 24h were immunostained with anti-TOMM20 antibody (green), anti-OPA1 antibody (red) and DAPI (blue); arrowheads indicate accumulation of OPA1 at the mitochondrial matrix (TOMM20). d, Quantification of OPA1 signal on TOMM20 positive structures from A549 cells illustrated in (e) (N = 30 cells from three independent experiments). Scale bars = 10μm. For evaluating significance of differences observed in b, d and f, a two-tailed Student’s t test was used (*** indicates p<0.0001).