posted on 2021-07-29, 17:27authored byShivangi Rastogi, Sarah Ellinwood, Jacques Augenstreich, Katrin D. Mayer-Barber, Volker Briken
BMDMs derived from wild type (WT), Aim2-/-, Ripk3-/-, and Ripk3-/-/Casp8-/- mice were either left uninfected (UI) or infected with CDC1551 Mtb and ΔpknF mutant at an MOI of 10 for 4h. The culture supernatants were harvested at 20 hpi and analyzed for (A, B) Secretion of IL-1β by ELISA and (C, D) cell death by quantification of the release of adenylate kinase (AK). Data are representative of three independent experiments. Error bars represent mean ± SEM; *, p<0.05, **, p<0.01, ns (non-significant).