Increased osteoclast formation from splenocytes of interferon-γ receptor knock-out (IFN-γR KO) mice

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Taken from "Enhanced osteoclast development in collagen-induced arthritis in interferon-γ receptor knock-out mice as related to increased splenic CD11bmyelopoiesis"

Arthritis Research & Therapy 2004;6(3):R220-R231.

Published online 12 Mar 2004

PMCID:PMC416444.

Copyright © 2004 De Klerck et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

Splenocytes of IFN-γR KO (KO) mice and wild-type (WT) mice were isolated. Mice were either naive or had been immunised with collagen type II in complete Freund's adjuvant 21 days previously. Cells were stimulated for 6 days in chamber slide cups with 20 ng/ml macrophage colony-stimulating factor (M-CSF) and 100 ng/ml receptor activator of NF-κB ligand (RANKL) or 20 ng/ml M-CSF and 20 ng/ml tumour necrosis factor-α (TNF-α) . After stimulation, cultures were fixed and stained for the presence of tartrate-resistant acid phosphatase (TRAP). (a,b) TRAPmultinucleated (three or more nuclei) cells were counted within each cup. In each group, bars represent averages ± standard error of the mean for five mice. *< 0.05 compared with wild–type mice (Mann-Whitney -test). Representative pictures of TRAP-stained cultures of RANKL-stimulated IFN-γR KO (c) and wild-type (d) splenocytes. Insets show details of multinucleated TRAPcells. Osteoclast activity after stimulation by RANKL of IFN-γR KO (e) and wild-type (f) splenocyte cultures, as analysed by their ability to resorb a calcium phosphate film coated on a quartz substrate. Sites of resorption are indicated by arrows. CIA, collagen-induced arthritis.