In the absence of miR-181c, lipogenesis is activated by IDH1 upregulation.

Western blot analysis of protein lysate from liver IDH1 (A) and IDH2 (B). (C) Quantitative polymerase chain reaction (qRT-PCR) was used to assess liver mRNA expression of genes involved in lipogenesis. Sterol regulatory element binding transcription factor 1 (SREBP1), fatty acid synthase (FASN), and ATP citrate lyase (ACLY) were more highly expressed in WT than in c/d KO mice. Data are expressed as mean ± SEM (n = 5-7/genotype/diet). *p<0.05 by t-test analysis after intergroup differences were found by 2-way ANOVA. *p<0.05 by two-sample t-test.