Impacts of antioxidants on lipid contents and HCV assembly.

(A) Huh-7 cells were transduced with guide RNAs and Cas9 and treated with puromycin for 7 days. Cell clones were isolated, and the level of ApoB was analyzed by western blotting. (B) The ApoB gene sequences of randomly selected clones were analyzed by Sanger sequencing. (C) Immunostaining was performed with an anti-ApoB antibody on ApoB^-/- cell clones. LDs were stained with BODIPY 493/503. Nuclei were stained with DAPI. Fluorescence signals were visualized by laser confocal microscopy. Scale bars, 10 μM. (D) ApoB^-/- cell clones were infected with HCV. Extracellular viral titers were analyzed. The data are shown as the means ± SDs of n = 3 biological repeats. The statistical significance was determined by unpaired two-sided Student’s t-tests. n.s., not significant.

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