pone.0256937.s001.tif (735.39 kB)
Impact of candidate iRPN13 upon high molecular weight polyubiquitinated protein levels and NFκB signaling.
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posted on 2021-09-10, 17:35 authored by Ravi K. Anchoori, Logan George, Ssu-Hsueh Tseng, Brandon Lam, Srinidhi Polkampally, Anjali D. Amiano, Palmer Foran, Hannah Tsingine, Harideep Samanapally, Fernanda Carrizo Velasquez, Samarjit Das, Deyin Xing, Ahmad Bin Salam, Balasubramanyam Karanam, Chien-Fu Hung, Richard B. S. Roden(A) HeLa cells treated with compounds (0.5 μM, 3h) show high molecular weight polyubiquitinated protein accumulation measured by Western blot analysis using anti-ubiquitin monoclonal antibody. (B) same as in A, except HCT116 cells were used (C) 293T cells stably transfected with a NFκB-dependent reporter luciferase construct show dose dependent reduction in bioluminescence with drug treatment (7 h) in response to TNFα (20 ng/mL) as measured by luminometer.
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xlink "> bortezomibproteasome &# 8217monomeric ubiquitin followingcombination approach warrantssynergistic cytotoxic responseirpn13s may compromiseubiquitin receptor rpn13evident upon treatmentcancer cell linesnew candidate irpn13sstress responseovarian cancercell lysatescandidate adrm1ubiquitination playssingle methylshow promiserapid accumulationprototypic inhibitorpotent analogsperinuclear clusteringoxidative stressmitochondrial depolarizationimproved bindingimportant roleearly biomarkercalled ra190asymmetry increasealternative non
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