Fig 4.tif (1.77 MB)
Immunofluorescence staining of Slc17a7 single KO and Slc17a7, Slc17a6, and Slc17a8 triple KO β-cell lines.
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posted on 2017-11-01, 17:42 authored by Naoya Murao, Norihide Yokoi, Kohei Honda, Guirong Han, Tomohide Hayami, Ghupurjan Gheni, Harumi Takahashi, Kohtaro Minami, Susumu SeinoGreen, Insulin; red, VGLUT1 (A) or VGLUT2 (B); blue, 4',6-diamidino-2-phenylindole (DAPI). Scale bars, 50 μm.
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Slc 17aGIPincretin-induced insulin secretionaspartate aminotransferase 1β- cell glutamatecytosolic glutamate productionpotentiate insulin secretionIIISpancreas perfusion experimentβ- cell linesMApancreatic β- cellsSlc 17a genesGLPASTglutamate transportersVGLUTSlc 17a KO miceinsulin granule exocytosisincretin-induced insulin secretion Incretinscytosolic glutamateSingle Slc 17a KO β- cell linesCRISPRwild-type Slc 17a
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