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Immunofluorescence images underlying figure 9 and figure 10 from paper Role of Aggregative Adherence Fimbriae
Images of mouse cecum and colon staining for EAEC bacteria by immunofluorescence
Methods: On day 4 for D5613, and day 5 post-infection for K411, mice were humanely euthanized as dictated by the approved protocol. Mouse ceca and colons were collected, fixed in formalin, embedded in paraffin, then further processed onto glass slides. For immunofluorescent detection of EAEC, unstained tissue sections on slides were deparaffinized with Histo-Clear (National Diagnostics), then treated with Antigen Unmasking Solution (Vector Laboratories) as per manufacturers’ protocols. Tissue sections were then incubated with 1:100 of anti-O154 (D5613) or anti-O128abc (K411) antibody from SSI diagnostica (Hillerød, Denmark) then with 1:200 goat anti-rabbit Alexa 488 (RRID: AB_2576217). As a counterstain for cell nuclei, SlowFade Diamond Antifade Mountant with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen) was used to mount coverslips.
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