ppat.1009355.s006.tif (1.14 MB)
Immunoblot analysis of CgPst2-CgYps1 interaction.
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posted on 2021-02-26, 12:51 authored by Anamika Battu, Rajaram Purushotham, Partha Dey, S. Surya Vamshi, Rupinder KaurCell extracts of wt and Cgyps1Δ strains expressing CgPst2-SFB (3 mg protein) were incubated with streptavidin beads for 2 h at 4οC and centrifuged at 2000 rpm for 5 min. Washed Beads were boiled in 2X SDS sample buffer, loaded on 8% (for CgYps1) and 12% (For SFB-tagged CgPst2) SDS-PAGE, and probed with anti-CgYps1 and anti-Flag antibodies, respectively. Please note that CgYps1 was not detectable in Input samples.
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arginine -174 residueCgPst 2.flavodoxin-like proteinyeast Candida glabrataimmunoprecipitation-mass spectromet...aspartyl protease-mediated cleavageCgYps 1-CgPst interactionaspartyl protease CgYps 1.implicating CgYps 1CgYps 1 protein levelsNADHstress response mechanismselectron-carrier flavodoxin-fold-co...CgPst 2aids oxidative stress survivalCgYps 1-mediated cleavageglycosylphosphatidylinositol-anchor...CgPst 2 cleavageoxidative stress defense system
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