Images showing the microscope setup for ToC cultures.

A. Global view of the Leica DMi8 used to perform the live imaging experiments. The white arrow points at the heating black chamber, in which is maintained a temperature of 37°C. The temperature is set and maintained by a temperature controller (blue arrow). The CO₂ controller (black arrow) mixes CO₂ with atmospheric air. Through a tubing system, the air with the controlled CO₂ at 5%, after passing through a bottle half filled with water for humidification (red arrow), is injected in the microscope chamber. B. View of the chip placed in the microscope chamber. The white arrow indicates the lid of the chamber in which is injected the humidified air with CO₂ at 5%. The red arrow points at the chip to be imaged. Humidified sponges contribute to humidify the chamber and to minimize micro-evaporation phenomena (black arrows). C. Picture from the side of the chip filled with medium. At each ‘anchor’ point, three magnets (red arrows) are used to lift and attach the chip to the glass slide (black arrow). D. Top view of an empty chip with magnets. Two piles composed of three magnets (red arrows) are applied in the central part of the chip and glass slide.

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