IgG subclass (IgG1-IgG4) responses against full-length PvCSP-VK210 and PvSSP3.

(A) IgG subclass responses to PvCSP-VK210 at acute phase among P. vivax-infected subjects (n = 35). (B-E) Longitudinal IgG1-4 subclass responses to PvCSP-VK210 in P. vivax-infected subjects (n = 10) at days 0, 90, 270 and 360. (F) IgG subclass responses to PvSSP3 at acute phase among P. vivax-infected subjects (n = 28). (G-J) Longitudinal IgG1-4 subclass responses to PvSSP3 in P. vivax subjects (n = 8) at days 0, 90, 270 and 360. The reactivity index (RI) was calculated by dividing mean OD value of each patient with cut-off value. Cut-off value was calculated from mean optical density (OD) values plus 2SD of 18 HCs for full-length PvCSP-VK210 and 14 HCs for PvSSP3. Black dots represent seropositive samples while white dots represent seronegative. The dashed line represents the cut-off value for seropositivity (RI = 1); samples were considered as seropositive if RI was greater than 1. Statistical testing was performed by one-way ANOVA: * p<0.05; ** p<0.005; *** p<0.0005; **** p<0.0001.