Identifications of host proteins that interact with the UTRs of the ANDV SmRNA.

(A) Depiction of the GRNA affinity chromatography combined with nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) strategy used to identify host proteins that interact with the 3’UTR of the ANDV SmRNA. (B) HeLa extracts precleared with 3BoxB, or 5’UTR-3BoxB RNA were incubated with different in vitro transcribed bait RNA. The RNA-proteins complexes were pulled down using recombinant λ-GST protein as described in [42]. The proteins bound to 3BoxB-3’UTR, 5’UTR-3BoxB, 5’UTR-3BoxB-3’UTR RNAs identified by Nano LC-MS/MS (Applied Biomics, Inc., Hayward, CA, USA) were grouped into four datasets (DS, the list of proteins can be found in S1 Data).