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Identification of chimeric RNAs by RT-PCR and Sanger sequencing.

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posted on 2023-12-04, 18:37 authored by Jun Pan, Shulin Wei, Qunnan Qiu, Xinyu Tong, Zeen Shen, Min Zhu, Xiaolong Hu, Chengliang Gong

A and B, fragments from the antisense RNAs of BmCPV S3 (A) and S4 (B) dsRNAs fused with a fragment of the larger subunit of ribosomal RNA (LSrRNA) from silkworm, the sequence indicated with a blue background is a common sequence shared by the S3/S4 and LSrRNA antisense sequences. C, Fragment of antisense RNA of BmCPV S7 dsRNA fused with a fragment of the silkworm mRNA clone: fcaL43P13. D, Fragment of silkworm U6 ncRNA fused with a fragment of sense RNA BmCPV S7 dsRNA. E and F, a fragment of silkworm U6 ncRNA/Bm_160 RNA fused with a fragment of the sense RNA of BmCPV S9 dsRNA. G, Fragment of antisense RNA of BmCPV S7 dsRNAs fused with a fragment of the antisense RNA of BmCPV S5 dsRNAs. H, A fragment of the sense RNA of BmCPV S9 dsRNAs fused with a fragment of the sense RNA of BmCPV S7 dsRNAs. The sequences indicated with a blue background are sequences shared by two parental RNAs.

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