IFN-γ, GrB and IL-10 production in response to parasite stimulation in individuals living in old versus recent ZCL foci.

IFN-γ (a), GrB (b) and IL-10 (c) were quantified by ELISA in the culture supernatants of PBMCs from donors residing in an old ZCL focus (MNA) or recent ZCL foci (MBA, KSR, MSD, DHB). The box represents the 25^th and 75^th percentiles. The median is represented by a solid horizontal line. The whiskers of the box show the 1^st percentile to the 99^th percentile. Statistically significant differences between the median of stimulated and non-stimulated cultures were assessed by the non-parametric median test. Results of the median test are shown. Statistical significance was assigned to a value of p<0,05.