IFN-γ, GrB and IL-10 production in response to parasite stimulation in individuals living in areas endemic for L. major infection (entire cohort).

IFN-γ (a), GrB (b) and IL-10 (c) were quantified by ELISA in the culture supernatants of PBMCs from donors residing in areas endemic for L. major infection. The supernatants were collected after 48h (for IL-10 detection) or 5 days (for IFN-γ and GrB detection) following stimulation with L. major parasites (5 parasites/cell). The box represents the 25^th and 75^th percentiles. The median is represented by a solid horizontal line. The whiskers of the box show the 1^st percentile to the 99^th percentile. Statistically significant differences between the median of stimulated and non-stimulated cultures were assessed by the non-parametric median test. Results of the median test are shown. Statistical significance was assigned to a value of p<0,05.