posted on 2021-11-09, 18:32authored bySteven A. Saenz, Andrea Local, Tiffany Carr, Arvind Shakya, Shivsmriti Koul, Haiqing Hu, Lisa Chourb, Justin Stedman, Jenna Malley, Laura Akullian D’Agostino, Veerabahu Shanmugasundaram, John Malona, C. Eric Schwartz, Lisa Beebe, Meghan Clements, Ganesh Rajaraman, John Cho, Lan Jiang, Alex Dubrovskiy, Matt Kreilein, Roman Shimanovich, Lawrence G. Hamann, Laure Escoubet, J. Michael Ellis
Concentrations of the RORγt inhibitors, Compound 1, Compound 2 or Compound 3, in which 50% (IC50) relative light units (RLUs) was inhibited. Assays utilized reporter cells (HEK293) harboring a receptor hybrid in which the native N-terminal DNA binding domain (DBD) has been replaced with that of the yeast Gal4 DBD, with a firefly luciferase reporter gene functionally linked to a Gal4 upstream activation sequence.