ppat.1009878.s001.tiff (7.3 MB)
Human PBMC are not permissive to SARS-CoV-2 infection.
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posted on 2021-09-02, 17:25 authored by Martina Severa, Roberta A. Diotti, Marilena P. Etna, Fabiana Rizzo, Stefano Fiore, Daniela Ricci, Marco Iannetta, Alessandro Sinigaglia, Alessandra Lodi, Nicasio Mancini, Elena Criscuolo, Massimo Clementi, Massimo Andreoni, Stefano Balducci, Luisa Barzon, Paola Stefanelli, Nicola Clementi, Eliana M. CocciaVirus titers were evaluated in viral inoculum (T0) as well as in peripheral blood mononuclear cell (PBMC) supernatants after 24 (T1) and 48 hours (T2) post SARS-CoV-2 infection in cultures treated at a multiplicity of infection (MOI) of 0.01, 0.02, 0.04 and 0.1. Shown results were calculated by Endpoint Dilution Assay by using the Reed-Muench formula and reported as TCID50/ml and derived from 3 experiments separately conducted.
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plasmacytoid dendritic cellsearly events resultingproductive viral replicationinhibiting viral replicationex vivo elevated inflammatory cytokineimmune cells occurringdiv >< pcytokine storm observedifn producing cellsifn response accompaniedpbmc enhances anti>, asymptomatic sarshigh serum typeimmune cellsviral responseviral ifnhigh levelsviral statevitro via inflammatory phenotypeinflammatory cytokinesifn secretedifn levelifn axisvigorous tlr7tissue damagestimulated genesshed lightorgan failurelow frequencyl1 markerkey roleinduced typeimpaired typeiii ifnshospitalized patientsearly interactiondependent productiondependent mechanismchemokine levelsassociated multi
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