HuCD3ε^HOM, but not huCD3ε^HET mice, show impaired thymocyte development.

(A) Representative histological staining (H&E) of thymi from WT, huCD3ε^HET, and huCD3ε^HOM mice. (B) Flow cytometry plots of murine thymocytes (gated on scatter profile and B220^-) comparing CD8⁺ x CD4⁺ thymocytes from each genotype. (C-D) Graphs showing frequencies (C) and absolute cell numbers (D) from (B). (E) Cell number ratio of CD4⁺CD8^- to CD4^-CD8⁺ thymocytes observed from each genotype. (F) Flow cytometry plots of murine thymocytes comparing CD25⁺ x CD44⁺ thymocytes. Cells are gated on scatter profile and B220^-. (G-H) Graphs showing frequencies (G) and absolute cell numbers (H) from (F). n = 3–5, *p<0.05.