HuCD3ε^HET peripheral T cells show normal phenotype.

(A) Representative flow cytometry plots of mouse splenocytes comparing CD62L⁺ x CD44⁺ expression on CD5⁺CD4⁺ (top row) or CD5⁺CD8⁺ (bottom row) WT, huCD3ε^HET and huCD3ε^HOM T cells. (B-E) Graphs summarizing the frequencies and absolute cell numbers of CD62L⁺CD44^- CD4⁺ or CD8⁺ T cells (B, D) and CD44⁺ CD4⁺ or CD8⁺ T cells (C, E) from each genetic background. (F) Graphical summary of CD69 expression on CD4⁺ or CD8⁺ T cells from naïve mice from each genotype. (G) Representative flow cytometry plots of apoptosis as measured by Ann-V x 7-AAD in fresh T cells of naïve mice. (H) Graphical summary of (G). n = 5–6, *p<0.05.