HuCD3ε-expressing T cells respond to anti-human CD3 binding.

(A) Splenocytes from WT and huCD3ε^HET were cultured in vitro for 24hrs with either anti-human (OKT3) or anti-murine (145-2C11) CD3, their respective isotype controls IgG2a or Armenian Hamster (AH), or anti-mouse TCRβ antibodies. CD69 expression was measured by flow cytometry. (B) Geometric mean fluorescence intensity of murine CD3ε as detected by flow cytometry on CD4⁺ and CD8⁺ T cells using 145-2C11 antibody. n = 6, *p<0.05. (C) Mice were administered an i.p. injection of either PBS, anti-mouse CD3 (clone 145-2C11), or anti-human CD3 (clone OKT3). Blood was collected 24hrs post-injection. CD5⁺ T cell numbers in peripheral blood were determined using CBC and flow cytometry. Individual animals are shown with group mean. n = 5, *p<0.05.