pntd.0011878.s015.tif (534.71 kB)
Histological observation (HE staining) was performed on Wistar rat corneas to study Acanthamoeba keratitis induced by intrastromal injection of amoebal trophozoites.
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posted on 2024-01-02, 19:15 authored by Binod Rayamajhee, Mark Willcox, Fiona L. Henriquez, Ajay Kumar Vijay, Constantinos Petsoglou, Gauri Shankar Shrestha, Hari Kumar Peguda, Nicole Carnt(A) and P. aeruginosa (B). Corneas infected by Acanthamoeba (AK10) alone (group C) showed a few areas of RBCs accumulation with the epithelium and endothelium slightly disorganized (A). In group D, the stromal region infected by P. aeruginosa exhibited a few necrosis like structures, RBCs, desquamated cells, inflammatory infiltrate, and visible disorganization of corneal epithelium and stromal structure (B). Indicators: White arrow, corneal epithelium; Yellow arrow: RBCs; Asterisk (*): Necrosis like structures with mild inflammatory infiltrate. Scale bar represents 15μm.
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primary human macrophagesincreasingly important pathogenwild type strainstrain pao1 (<increased neutrophil responsealkyne – functionalizednaturally acquired intracellulardomestic tap waterpseudomonas aeruginosa live intracellular bacteriarats eyes resulteddomestic watermotile strainaeruginosa – alanineclinical responsehousehold waterintracellular survivalintracellular bacteriavivo situ p acanthamoeba various microorganismsspecies recoveredseen throughoutpotentially attributedmetabolically activemay indicategreater degreegenotype t4ffish ),findings indicateenvironmental hostelectron microscopycorneal swabscorneal infectionschallenged intrastromallyanimal modelalso becomingalkdala ).ak patients>< 005 ).
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