pone.0261853.g003.tif (393.55 kB)
High interlaboratory agreement in Ct values for blinded clinical samples in Project B.
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posted on 2022-01-13, 18:44 authored by Margaret G. Mills, Emily Bruce, Meei-Li Huang, Jessica W. Crothers, Ollivier Hyrien, Christopher A. L. Oura, Lemar Blake, Arianne Brown Jordan, Susan Hester, Leah Wehmas, Bernard Mari, Pascal Barby, Caroline Lacoux, Julien Fassy, Pablo Vial, Cecilia Vial, Jose R. W. Martinez, Olusola Olalekan Oladipo, Bitrus Inuwa, Ismaila Shittu, Clement A. Meseko, Roger Chammas, Carlos Ferreira Santos, Thiago José Dionísio, Thais Francini Garbieri, Viviane Aparecida Parisi, Maria Cassia Mendes-Correa, Anderson V. de Paula, Camila M. Romano, Luiz Gustavo Bentim Góes, Paola Minoprio, Angelica C. Campos, Marielton P. Cunha, Ana Paula P. Vilela, Tonney Nyirenda, Rajhab Sawasawa Mkakosya, Adamson S. Muula, Rebekah E. Dumm, Rebecca M. Harris, Constance A. Mitchell, Syril Pettit, Jason Botten, Keith R. JeromeCt values plotted against the sample-specific average Ct values from the 10 laboratories. The solid line indicates the line of equation y = x. Each color indicates one laboratory; the same color is used to identify each laboratory in all subsequent figures.
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viral transport mediapurified nucleic acidsgenerate actionable datafewer experimental resourcesless &# 8221oral pharyngeal samplesconcordance across laboratoriesaccessible qualitative methodless &# 8220direct &# 8221pcr assay methods&# 8220participating laboratoriesnegative samplesdirect rtresults suggestreliable detectionqpcr methodpotently inhibitpcr reactionobserved 100lower costhouse equipmentfeasible optionequipment shortagesefficient covidcorrect identificationcontain charcoalcommon reagentscommon protocol2 rna2 detection2 ).
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