1471-2164-9-68-3.jpg (30.76 kB)
High-throughput mammalian two-hybrid screening for protein-protein interactions using transfected cell arrays-2
figure
posted on 2011-12-31, 13:59 authored by Andrea Fiebitz, Lajos Nyarsik, Bernard Haendler, Yu-Hui Hu, Florian Wagner, Sabine Thamm, Hans Lehrach, Michal Janitz, Dominique VanheckeCopyright information:
Taken from "High-throughput mammalian two-hybrid screening for protein-protein interactions using transfected cell arrays"
http://www.biomedcentral.com/1471-2164/9/68
BMC Genomics 2008;9():68-68.
Published online 6 Feb 2008
PMCID:PMC2254387.
st and antagonists. : Dose-dependent induction of AR-LBD and AR-NTD interaction by the synthetic agonist R1881, showing a maximal response from 10 nM onwards. : Dose-dependent inhibition of R1881 (10 nM) induced AR-LBD and AR-NTD interaction by two antagonists, medroxyprogesterone acetate (MPA) and hydroxyflutamide (OH-Flu). Quantitative analysis of this inhibition reflects the stronger antagonistic potency of MPA which can achieve maximum inhibition at a concentration of 1 nM as compared to OH-Flu, of which more than 10 nM is required to obtain the same level of inhibition. Data shown represents the mean fluorescence of 6 features per sample, collected from triplicate spots on two identical slides.