Heterogeneity of GBS subtypes based on EssC variation and putative T7SS effectors.

a) Percent identity matrix of full EssC amino acid sequences from example GBS, S. aureus, and L. monocytogenes strains from various T7SS subtypes. Strain information is listed in S2 Table. The purple shading corresponds to the level of identity between two strains (on a spectrum of 0 to 100% identity), with darker shading indicative of higher percent identity. b) Diagram of putative T7SS loci from example strains across GBS T7SS subtypes I—IV. CJB111 (accession CP063198.2) is an example of subtype I and expresses the EssC1 variant (ID870_04200). 2603 V/R (accession NC_004116.1) is an example of subtype II and expresses the EssC2 variant (SAG_RS07895). CNTC 10/84 (accession NZ_CP006910.1) is an example of subtype III and expresses the EssC3 variant (W903_RS05455). COH1 (accession NZ_HG939456.1) is an example of subtype IV and expresses the EssC4 variant (GBSCOH1_RS05095). Genes in purple encode WXG100 or WXG100-like proteins, gene in teal encodes a LXG domain-containing protein, and gene in maroon encodes a DUF4176 domain-containing protein. Genes in various shades of gray are either annotated as hypothetical or do not have a predicted function. Arrows with patterns indicate T7SS subtype-specific genes that exhibit little to no homology to those present in other GBS T7SS subtypes. Putative core genes of the operon are esaA through essC.

(TIF)