Hepatocyte viability is maintained over study period.

(A)There was no significant difference in cell viability between LA and control groups at any time based on luminescence measurements from the ATP quantification assay (n = 3/group). (B) Hepatic functionality, as measured by urea, was not impacted by LA feeding over 3 days of culture. Maximum urea levels were detected 24 hours post-feeding in both LA and HSA-control groups, indicating that the constructs were capable of secreting urea up to baseline levels following LA or HSA exposure. (C) The expression levels of CYP1A1, a member of the cytochrome P450 family, were not significantly impacted by feeding with LA or HSA when compared to pre-feeding levels.